二十年來 Gene Construction Kit(GCK) 一直是許多專業研究人員描繪DNA的首選軟體。GCK可以輕鬆控制DNA的排序，無論是圖形或文本序列，如此一來便可大量減少花費的時間和成本。
GCK eliminates tedious examination of DNA sequence data by automatically identifying open reading frames (ORF's), keeping track of sticky ends during cutting and pasting of restriction enzyme digestion fragments, assisting with PCR primer design, and enabling comprehensive annotation of DNA sequence features.
這套DNA分析軟體可以同時打開多個文件查看，輕鬆地在質體和向量間複製、貼上DNA序列，呈現出真實世界的 DNA cloning protocols。
Gene Construction Kit軟體可在Windows和Macintosh系統上使用，文件檔案也可以跨平台共享。GCK的使用者介面和和功能設定可以顯著提高實驗室的效率並減少實驗設計錯誤，大幅降低花費的時間與成本。
Gene Construction Kit 4.5 is supported under Mac OS 10.10- 10.13 (High Sierra); as well as Windows 10 (thru version 1607), 8, & 7.
flexible formatting & manipulation
DNA can be represented and manipulated in either graphical or sequence/text modes - with any formatting or sequence manipulation changes made in one view, automatically being applied to the selection in the alternate view. Users have complete control over the display in either mode. Segments can be selected in either mode and cut, copied, or pasted. When a selected segment is cut or copied, the ends are defined by the sites used to delimit the segment. Gene Construction Kit automatically monitors sticky ends that can be generated by restriction enzymes. Incompatible ends cannot inadvertently be pasted together. If such an attempt is made, GCK utilizes a unique "ligation dialog" to assist users in adjusting the ends for compatibility. Utilizing the special paste option allows a sequence to be inverted and/or to have the ends filled or trimmed before pasting, thus circumventing the ligation dialog if the manipulations warrant it.
Gene Construction Kit provides users with lists containing all known restriction enzymes with which to work, while also providing lists containing only commercial enzymes or enzymes sorted by recognition sequence lengths. Users can choose to mark enzyme sites by how many times they cut a sequence, by the kind of cut they create (5', 3', blunt), or even by whether or not they cut within a specified segment. One can even generate a table output of 'No cutters' for a specific selection of DNA. This flexibility allows sophisticated searching - such as marking all unique blunt end cutters that cut only within a selected segment of DNA; or - marking all enzymes that cut more than 5 times, but only cut outside a selected segment.
customized GenBank conversion
From within Gene Construction Kit users can directly download a GenBank entry if an accession number is known - or one can search GenBank for keyword matches in all the relevant fields (Author, Gene Name, Journal, Organism, etc). Either method produces a panel showing matching entries, the features identified, and the source information contained within the field.
intuitive analysis tools
find open reading frames (ORF's)
GCK allows users to identify open reading frames based upon user defined parameters. The minimum ORF length can be defined, as well as whether the program should search one or both strands - or whether every ORF should begin with the bases 'ATG'. Additionally, users can change the codon table used in the analysis - should the sequence warrant a different table from the default.
integrated project management
A unique, and very useful feature in GCK is called 'Chronography.' This feature allows a user to keep track of construct history as well as to store different views of the same sequence in a single file. Although one will be seeing different graphics on screen, there is only a single sequence in each construct file. Users can create up to 32,000 generations for any construct - making it virtually limitless. A user might choose to store a generation of a construct displaying restriction sites, another generation could be defined for the coding regions, another the transcripts, and yet a fourth generation might show the sources of each DNA in the construct. The ability to show any generation for any segment and to mix generations in viewing the same construct, provides a great deal of flexibility in displaying the information that is part of the construct while at the same time providing a natural way to keep track of the history of complex constructions. One never loses track of where a specific piece of DNA came from since previous generations of that segment are always available for viewing through chronography. When copying and pasting, the different chronography generations are copied along with the sequence itself. Chronography allows the flexibility of displaying the same sequence in a number of ways to bring out key features.
Gene Inspector (GI)是一個集合電子實驗室筆記本、完整DNA和蛋白質序列分析套裝和強大的繪圖工具於一體的多功能軟體。
Intel compatible Pentium class processor
Windows 8, 7, Vista or XP SP3
Intel Core Duo processor
Mac OS 10.8 (Mountain Lion), 10.7 (Lion) & 10.6 (Snow Leopard)
Technical Requirements Under Both OS’s
250 MB RAM*
50 MB of available hard disk space
Internet connection required for GenBank/EMBL & Database Searches
* RAM requirements vary depending upon the size of data being analyzed.
powerful integrated e-book
Gene Inspector is centered around an electronic notebook which can contain many kinds of objects, including analysis results. Output from the various analyses in Gene Inspector are placed into the notebook as they are run, showing users the results and allowing annotations directly on the page. Text wraps around notebook objects and can be used to discuss experimental results just as in a paper lab notebook.
customizable dna and protein analysis
Gene Inspector features a standard interface for setting up and running any of the more than 40 built-in DNA and protein analyses. This straight-forward interface allows users to spend more time analyzing results rather than deciphering the software manual. Analyses are first chosen from a scrollable list. As an item in the list is selected, "help" information appears next to the selected analysis describing that analysis.
flexible multiple sequence editor
Gene Inspector offers a sophisticated sequence editor which can accommodate single, or multiple DNA or protein sequences. Individual sequences can be colored and each sequence is interlaced with the others to allow easy comparison. An overview "pane" at the top of this window shows which segment(s) is being displayed in the sequence pane and can be used to navigate to specific locations in specific sequences. Any number of individual sequences may be placed into a single sequence document - allowing users to save a single file that contains all sequences used in a project.
built-in time savings
Often a researcher will be working on a sequence that is constantly being refined. As new information about the sequence comes to light, there is a need to repeat all the analyses based on that sequence. Gene Inspector has the ability to "hotlink" an analysis to a sequence. This means that when a sequence is changed, all dependent analyses can be updated automatically. There is no need to go back and re-define parameters and re-run each analysis again. The program highlights 'outdated' analyses with a yellow circle containing an exclamation point - quickly showing the user that the underlying sequence information has been changed since this analysis was last performed. Users can simply click to 'Perform Auto Recalc Now' from the Notebook menu to update all out of date analyses.
Hotlinks can be very useful - for example a user can create an entire notebook containing many analyses all linked to a single sequence. To perform this set of analyses on a new sequence, just paste the new sequence in place of the original sequence and perform the auto recalc to update.
Gene Inspector's notebook is effectively used to track experiments and discuss results - but with the graphic and layout capabilities, it also becomes a valuable tool for designing posters and presentations. The notebook layout can be changed to any dimension containing any number of text columns per sheet so your experimental results can be readily converted into a poster, slide, or manuscript figure.
Arrows and other graphic objects can be placed in the notebook to highlight critical points, like the peaks on the transmembrane helix, or a candidate open reading frame of a codon preference plot. Different backgrounds can be defined for output objects and frames (with or without shadows) can be added. Images from other sources, such as scanned gel images, or cloning strategies copied from a Gene Construction Kit illustration can be pasted into the notebook page for reference.
The background text in a Gene Inspector notebook can be "styled" as conditional text and then either shown or hidden. Thus, only the text you want to see will be shown when making the illustration. This enables the Gene Inspector notebook to serve as a daily workbook and also to be used as a presentation tool.