Molecular Cloning

• Restriction Cloning
• Gateway Cloning
• Gibson Assembly
• NEBuilder HiFi Assembly
• In-Fusion Cloning
• TA & GC Cloning
• TOPO Cloning

Primers

• Design primers
• Anneal two oligos to form a double-stranded product

PCR and Mutagenesis

• Simulate PCR
• Overlap extension PCR
• Primer-directed mutagenesis

Enzyme Sets

• Predefined enzyme sets - by company or cutter
• Create custom enzyme sets
• View detailed enzyme information
• Rich support for methylation sensitivity and associated error prevention

Convert File Formats

• Alignment Formats
• ApE
• CLC Bio
• Clone Manager
• DNA Strider
• DNADynamo
• DNASIS
• DNAssist
• DNASTAR Lasergene®
• DS Gene
• EMBL (ENA)
• EnzymeX
• GenBank / DDBJ
• Gene Construction Kit®
• Geneious
• GeneTool
• Genome Compiler
• Jellyfish
• MacVector
• pDRAW32
• Serial Cloner
• Swiss-Prot
• Vector NTI®
• Visual Cloning

Agarose Gel Simulation

• Simulate an agarose gel
• Simulate a Restriction Digest
• Simulate a PCR Amplification
• Large collection of MW markers

Features / Annotations

• Create and edit features
• Automatic annotation of common features
• Annotate novel features manually
• Choose Alternative Codons
• Sophisticated numbering of feature translations
• Support for ribosomal slippage

Translations

• View and edit translated features
• Open reading frames (ORFs)
• Whole-sequence translations
• Check reading frames for gene fusions
• Make Protein (from DNA)
• Reverse Translate (from Protein)

Alignment

• Align DNA sequences with a reference sequence
• Verify cloning or mutagenesis
• Align cDNA to a chromosome
• Pairwise and multi sequence DNA and Protein alignment
• Choice of alignment algorithms - Clustal Omega, MAFFT, MUSCLE, T-Coffee
• Contig Assembly

Visualizing

• See multiple views of a DNA sequence
• Large sequence support - browse chromosome size sequences
• Edit DNA and protein sequences
• Color code sequences

History Tracking

• Comprehensive “undo” capability
• See a graphical history of a product
• Use optional history colors to identify the most recent change to a sequence

Data Management

• Import from common file formats including annotations and notes
• Export to standard formats
• Create and share Collections
• Share data with SnapGene Viewer
• Run batch operations

Search

• Search for DNA or protein sequences
• Search for enzymes, features or primers

General
Cross platform compatibility - Windows, macOS, Linux